TRIM33 antibody (AA 1001-1127)

Catalog No. ABIN3043492

Product Details anti-TRIM33 Antibody

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Binding Specificity: AA 1001-1127

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-TIF1 gamma/TRIM33 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TIF1 gamma/TRIM33 Antibody Picoband® (ABIN3043492). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TIF1 gamma recombinant protein (Position: M1001-K1127). Human TIF1 gamma shares 96.1% amino acid (aa) sequence identity with mouse TIF1 gamma.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: TRIM33 tripartite motif-containing 33". 2. He, W., Dorn, D. C., Erdjument-Bromage, H., Tempst, P., Moore, M. A. S., Massague, J. Hematopoiesis controlled by distinct TIF1-gamma and Smad4 branches of the TGF-beta pathway. Cell 125: 929-941, 2006. 3. Venturini, L., You, J., Stadler, M., Galien, R., Lallemand, V., Koken, M. H. M., Mattei, M. G., Ganser, A., Chambon, P., Losson, R., de The, H. TIF1-gamma, a novel member of the transcriptional intermediary factor 1 family. Oncogene 18: 1209-1217, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TRIM33

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Alternative Name: TRIM33 (TRIM33 Products)

Background:

Synonyms: E3 ubiquitin-protein ligase TRIM33,6.3.2.-,Ectodermin homolog,RET-fused gene 7 protein,Protein Rfg7,Transcription intermediary factor 1-gamma,TIF1-gamma,Tripartite motif-containing protein 33,TRIM33,KIAA1113, RFG7, TIF1G,

Tissue Specificity: Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors.

Background: Tripartite motif-containing 33 (TRIM33), also known as transcriptional intermediary factor 1 gamma (TIF1-γ), is a human gene. The TRIM33 gene is mapped to chromosome 1p13 by FISH. The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Molecular Weight: 150 kDa

Gene ID: 51592