HRPT2 antibody (AA 119-520)

Catalog No. ABIN3043535

The Rabbit Polyclonal anti-HRPT2 antibody has been validated for WB. It is suitable to detect HRPT2 in samples from Human.

Quick Overview for HRPT2 antibody (AA 119-520) (ABIN3043535)

Target: HRPT2 (CDC73) (Parafibromin (CDC73))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRPT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HRPT2 Antibody

Binding Specificity: AA 119-520

Purpose: Anti-HRPT2/CDC73 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HRPT2/CDC73 Antibody Picoband® (ABIN3043535). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HRPT2 recombinant protein (Position: Q119-R520). Human HRPT2 shares 100% and 99.8% amino acid (aa) sequence identity with mouse and rat HRPT2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: CDC73 Cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)". 2. Sood R, Bonner TI, Makalowska I, Stephan DA, Robbins CM, Connors TD, Morgenbesser SD, Su K, Faruque MU, Pinkett H, Graham C, Baxevanis AD, Klinger KW, Landes GM, Trent JM, Carpten JD (April 2001). "Cloning and characterization of 13 novel transcripts and the human RGS8 gene from the 1q25 region encompassing the hereditary prostate cancer (HPC1) locus". Genomics 73 (2): 211-22.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HRPT2

Target: HRPT2 (CDC73) (Parafibromin (CDC73))

Alternative Name: CDC73

Background:

Synonyms: Parafibromin,Cell division cycle protein 73 homolog,Hyperparathyroidism 2 protein,CDC73,C1orf28, HRPT2,

Tissue Specificity: Found in adrenal and parathyroid glands, kidney and heart. .

Background: Cell division cycle 73, also known as HRPT2, is a protein which in humans is encoded by the CDC73 gene. This gene encodes a tumor suppressor that is involved in transcriptional and post-transcriptional control pathways. The protein is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. And this protein appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in this gene have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma.

Sequence Similarities: Belongs to the CDC73 family.

Molecular Weight: 61 kDa

Gene ID: 79577

UniProt: Q6P1J9

Pathways: Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance