PKC alpha antibody (AA 153-342)

Catalog No. ABIN3043549

This Rabbit Polyclonal antibody specifically detects PKC alpha in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for PKC alpha antibody (AA 153-342) (ABIN3043549)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PKC alpha Antibody

Binding Specificity: AA 153-342

Purpose: Anti-PKC alpha/PRKCA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PKC alpha/PRKCA Antibody Picoband® (ABIN3043549). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PKC alpha recombinant protein (Position: M153-L342). Human PKC alpha shares 97% and 99% amino acid (aa) sequence identity with mouse and rat PKC alpha, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat1. Braz, J. C., Gregory, K., Pathak, A., Zhao, W., Sahin, B., Klevitsky, R., Kimball, T. F., Lorenz, J. N., Nairn, A. C., Liggett, S. B., Bodi, I., Wang, S., and 9 others : PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nature Med. 10: 248-254, 2004. 2. Latos-Bielenska, A., Klett, C., Just, W., Hameister, H. : Refinement of localization of the human genes for myeloperoxidase (MPO), protein kinase C, alpha polypeptide, PRKCA, and the DNA fragment D17S21 on chromosome 17q. Hereditas 115: 69-72, 1991.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-PKC alpha antibody (ABIN3043549)

Yan-Fang, Zhi-Heng, Li-Xiao, Fang, Jun, Gang, Lan, Na-Na, Xiao-Juan, Li-Chao, Wen-Li, Pei-Fang, He, Guang-Hao, Yan-Hong, Yi-Ping, Yun-Yun, Hui-Ting, Yi, Mei-Fang, Lin, Jian, Shao-Yan, Xue-Ming, Xing et al.: "Molecular Mechanism of the Cell Death Induced by the Histone Deacetylase Pan Inhibitor LBH589 (Panobinostat) in Wilms Tumor Cells. ..." in: PLoS ONE, Vol. 10, Issue 7, pp. e0126566, (2016) (PubMed).

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Cheng, Gu, Zhang, Yuan, Zhao, Jiang, Jia: "Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-?-ERK1/2-NF-?B pathway." in: International immunopharmacology, Vol. 23, Issue 1, pp. 304-13, (2015) (PubMed).

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA

Background:

Synonyms: Protein kinase C alpha type,PKC-A,PKC-alpha,2.7.11.13,PRKCA,PKCA, PRKACA,

Tissue Specificity: Ubiquitous. Isoform 2 is lymphocyte-specific.

Background: Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.

Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Molecular Weight: 77 kDa

Gene ID: 5578

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling