DLG1 antibody (AA 1-165)

Catalog No. ABIN3043562

The Rabbit Polyclonal anti-DLG1 antibody has been validated for WB and IHC. It is suitable to detect DLG1 in samples from Human, Rat and Mouse.

Quick Overview for DLG1 antibody (AA 1-165) (ABIN3043562)

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DLG1 Antibody

Binding Specificity: AA 1-165

Purpose: Anti-SAP97/DLG1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SAP97/DLG1 Antibody Picoband® (ABIN3043562). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SAP97 recombinant protein (Position: M1-A165). Human SAP97 shares 89.7% and 84.2% amino acid (aa) sequence identity with mouse and rat SAP97, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Müller BM, Kistner U, Veh RW et al. (March 1995). "Molecular characterization and spatial distribution of SAP97, a novel presynaptic protein homologous to SAP90 and the Drosophila discs-large tumor suppressor protein". J. Neurosci. 15 (3 Pt 2): 2354-66. 2. umbaugh G, Sia GM, Garner CC, Huganir RL (June 2003). "Synapse-associated protein-97 isoform-specific regulation of surface AMPA receptors and synaptic function in cultured neurons". J. Neurosci. 23 (11): 4567-76. 3. Sans N, Racca C, Petralia RS, Wang YX, McCallum J, Wenthold RJ (October 2001). "Synapse-associated protein 97 selectively associates with a subset of AMPA receptors early in their biosynthetic pathway". J. Neurosci. 21 (19): 7506-16.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DLG1

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Alternative Name: DLG1

Background:

Synonyms: Disks large homolog 1,Synapse-associated protein 97,SAP-97,SAP97,hDlg,DLG1,

Tissue Specificity: Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles. .

Background: Disks large homolog 1 (DLG1), also known as synapse-associated protein 97 or SAP97, is a protein that in humans is encoded by the SAP97 gene. SAP97 is expressed throughout the body in epithelial cells, including the kidney and brain. There is some evidence that SAP97 regulates cell-to-cell adhesion during cell death, and may interact with HPV. In the brain, SAP97's function is involved in the trafficking of transmembrane receptors from the ER to the plasma membrane. SAP97's function has been investigated by reducing its expression by knockout or increasing its expression heterologously. Mice in which the SAP97 gene has been knocked out die perinatally, have a cleft palate, and deficiencies in renal function. Overexpression of SAP97 in mammalian neurons leads to increased synaptic strength.

Sequence Similarities: Belongs to the MAGUK family.

Molecular Weight: 130 kDa

Gene ID: 1739

UniProt: Q12959

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response