PFN1 antibody (C-Term)

Catalog No. ABIN3043603

This Rabbit Polyclonal antibody specifically detects PFN1 in WB, IHC, ICC, FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PFN1 antibody (C-Term) (ABIN3043603)

Target: PFN1 (Profilin 1 (PFN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-PFN1 Antibody

Binding Specificity: AA 116-140, C-Term

Purpose: Anti-Profilin 1/PFN1 Antibody Picoband®

Sequence: KEGVHGGLIN KKCYEMASHL RRSQY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Profilin 1/PFN1 Antibody Picoband® (ABIN3043603). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: PFN1 profilin 1" 2. Kwiatkowski, D. J., Aklog, L., Ledbetter, D. H., Morton, C. C. Identification of the functional profilin gene, its localization to chromosome subband 17p13.3, and demonstration of its deletion in some patients with Miller-Dieker syndrome. Am. J. Hum. Genet. 46: 559-567, 1990. 3. Mockrin, S. C., Korn, E. D. Acanthamoeba profilin interacts with G-actin to increase the rate of exchange of actin-bound adenosine 5-prime-triphosphate. Biochemistry 19: 5359-5362, 1980.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PFN1

Target: PFN1 (Profilin 1 (PFN1))

Alternative Name: PFN1

Background:

Synonyms: Profilin-1,Epididymis tissue protein Li 184a,Profilin I,PFN1,

Tissue Specificity: Expressed in epididymis (at protein level). .

Background: Profilin-1 is a protein that in humans is encoded by the PFN1 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. PFN1 is mapped to 17p13.2. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. This gene is a 140-amino acid protein and major growth regulator of filamentous (F)-actin through its binding of monomeric (G)-actin. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Sequence Similarities: Contains 1 PAC (PAS-associated C-terminal) domain.

Molecular Weight: 15 kDa

Gene ID: 5216

UniProt: P07737

Pathways: Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location