MBP antibody (C-Term)

Catalog No. ABIN3043688

The Rabbit Polyclonal anti-MBP antibody has been validated for WB, IHC and IF. It is suitable to detect MBP in samples from Rat and Mouse. There are 13+ publications available.

Quick Overview for MBP antibody (C-Term) (ABIN3043688)

Target: MBP

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MBP Antibody

Binding Specificity: AA 182-197, C-Term

Purpose: Anti-Myelin Basic Protein/MBP Antibody Picoband®

Sequence: KLGGRDSRSG SPMARR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Myelin Basic Protein/MBP Antibody (ABIN3043688). Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunofluorescence, 2 μg/mL, Mouse, Rat
1. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D, Pessac B.The myelin basic protein gene is expressed in differentiated blood cell lineages and in hemopoietic progenitors. Proc Natl Acad Sci U S A. 2002 Jun 25, 99(13):8856-61. 2. Streicher R, Stoffel W.The organization of the human myelin basic protein gene. Comparison with the mouse gene. Biol Chem Hoppe Seyler. 1989 May, 370(5):503-10. 3. McLaurin JA, Hafler DA, Antel JP.Reactivity of normal T-cell lines to MBP isolated from normal and multiple sclerosis white matter. J Neurol Sci. 1995 Feb, 128(2):205-11. 4. Iester A, Vignola S, Callegarini L, Gimelli G, Alpigiani MG. [18q syndrome with deficiency of myelin basic protein (MBP)] Pediatr Med Chir. 1996 Mar-Apr, 18(2):201-5. 5. Tranquill LR, Skinner E, Campagnoni C, Vergelli M, Hemmer B, Muraro P, Martin R, McFarland HF, Campagnoni AT, Voskuhl RR. Human T lymphocytes specific for the immunodominant 83-99 epitope of myelin basic protein: recognition of golli MBP HOG 7. J Neurosci Res. 1996 Sep 15, 45(6):820-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.01 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-MBP antibody (ABIN3043688)

Zhou, Liu, Wang, Zou, Wei, Zhou: "Neuroprotective effect of ischemic postconditioning on sciatic nerve transection." in: Neural regeneration research, Vol. 13, Issue 3, pp. 492-496, (2018) (PubMed).

Mi, Gao, Liu, Ye, Li, Jin, Yang, Yang: "Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 20, pp. 2780-91, (2017) (PubMed).

Hu, Zhang, Tian, Xun, Wang, Lv: "Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 49-58, (2016) (PubMed).

Jin, Liu, Deng, Liao, Lin, Ning, Luo et al.: "Neuroprotective effects of activated protein C on intrauterine inflammation-induced neonatal white matter injury are associated with the downregulation of fibrinogen-like protein 2/fibroleukin ..." in: International journal of molecular medicine, Vol. 35, Issue 5, pp. 1199-212, (2015) (PubMed).

Chen, Chen, Huang, Qin, Fang, Liu, Zhang, Pan, Wang, Xie: "Soluble epoxide hydrolase inhibition provides multi-target therapeutic effects in rats after spinal cord injury." in: Molecular neurobiology, (2015) (PubMed).

Zhang, Chai, Liu, Xu, Yang: "Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1141-1148, (2015) (PubMed).

Yang, Gao, Zhang, Fang, Wu, Xu, Huang: "Changes in proinflammatory cytokines and white matter in chronically stressed rats." in: Neuropsychiatric disease and treatment, Vol. 11, pp. 597-607, (2015) (PubMed).

Gao, Zheng, Cai, Yao, Wang, Zhang, Wang: "Comparison of morphology and biocompatibility of acellular nerve scaffolds processed by different chemical methods." in: Journal of materials science. Materials in medicine, Vol. 25, Issue 5, pp. 1283-91, (2014) (PubMed).

Han, Wan, Kudo, Foltz, Vines, Green, Zoerle, Tariq, Brathwaite, DAbbondanza, Ai, Macdonald: "Molecular alterations in the hippocampus after experimental subarachnoid hemorrhage." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 34, Issue 1, pp. 108-17, (2014) (PubMed).

Wei, Zheng, Liang, Wei, Yin, Tang, Xue: "Apolipoprotein E and its mimetic peptide suppress Th1 and Th17 responses in experimental autoimmune encephalomyelitis." in: Neurobiology of disease, Vol. 56, pp. 59-65, (2013) (PubMed).

Wang, Tu, Zhao, An, Wang, Liu, Yuan, Ahmed, Xu, Liu, Zhang, Wang: "Effects of poly(L-lysine)-modified Fe3O4 nanoparticles on endogenous reactive oxygen species in cancer stem cells." in: Biomaterials, Vol. 34, Issue 4, pp. 1155-69, (2012) (PubMed).

Ping, Yao, Jiang, Zhao, Yu, Jiang, Lin, Chen, Wang, Zhang, Cui, Qian, Wang, Bian: "The chemokine CXCL12 and its receptor CXCR4 promote glioma stem cell-mediated VEGF production and tumour angiogenesis via PI3K/AKT signalling." in: The Journal of pathology, Vol. 224, Issue 3, pp. 344-54, (2011) (PubMed).

Wang, Xiao, Chen, Han, Gao, Zhang, Zhao, Wang, Dai: "Nogo-66 promotes the differentiation of neural progenitors into astroglial lineage cells through mTOR-STAT3 pathway." in: PLoS ONE, Vol. 3, Issue 3, pp. e1856, (2008) (PubMed).

Target Details for MBP

Target: MBP

Background:

Synonyms: Myelin basic protein,MBP,Myelin A1 protein,Myelin membrane encephalitogenic protein,MBP,

Tissue Specificity: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. .

Background: Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Sequence Similarities: Belongs to the myelin basic protein family.

Molecular Weight: 17-33 kDa

UniProt: P02686