XAF1 antibody (C-Term)

Catalog No. ABIN3043705

This Rabbit Polyclonal antibody specifically detects XAF1 in WB, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for XAF1 antibody (C-Term) (ABIN3043705)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-XAF1 Antibody

Binding Specificity: AA 283-301, C-Term

Purpose: Anti-XIAP-associated factor 1 XAF1 Antibody Picoband®

Sequence: QEKCRWLASS KGKQVRNFS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-XIAP-associated factor 1 XAF1 Antibody (ABIN3043705). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fong, W. G., Liston, P., Rajcan-Separovic, E., St. Jean, M., Craig, C., Korneluk, R. G. : Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 70: 113-122, 2000. 2. Liston, P., Fong, W. G., Kelly, N. L., Toji, S., Miyazaki, T., Conte, D., Tamai, K., Craig, C. G., McBurney, M. W., Korneluk, R. G. : Identification of XAF1 as an antagonist of XIAP anti-caspase activity. Nature Cell Biol. 3: 128-133, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XAF1

Background:

Synonyms: XIAP-associated factor 1,BIRC4-binding protein,XAF1,BIRC4BP, XIAPAF1,

Tissue Specificity: Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). .

Background: XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis (XIAP, MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Sequence Similarities: Contains 1 TRAF-type zinc finger.

Molecular Weight: 35 kDa