Aquaporin 5 antibody (C-Term)

Catalog No. ABIN3043712

The Rabbit Polyclonal anti-Aquaporin 5 antibody has been validated for WB and IHC. It is suitable to detect Aquaporin 5 in samples from Mouse and Rat. There are 2+ publications available.

Quick Overview for Aquaporin 5 antibody (C-Term) (ABIN3043712)

Target: Aquaporin 5 (AQP5)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Aquaporin 5 Antibody

Binding Specificity: AA 246-265, C-Term

Purpose: Anti-Aquaporin 5/AQP5 Antibody Picoband®

Sequence: EEDWEDHREE RKKTIELTAH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Aquaporin 5/AQP5 Antibody (ABIN3043712). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse AQP5, identical to the related rat sequence.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Rat, Mouse
1. Verkman AS (2003). "Role of aquaporin water channels in eye function.". Exp. Eye Res. 76 (2): 137-43. 2. Ma T, Yang B, Umenishi F, Verkman AS (1997). "Closely spaced tandem arrangement of AQP2, AQP5, and AQP6 genes in a 27-kilobase segment at chromosome locus 12q13.". Genomics 43 (3): 387-9.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Aquaporin 5 antibody (ABIN3043712)

Guo, Kazadaeva, Ortega, Manjunath, Desai: "Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung." in: Developmental biology, Vol. 430, Issue 1, pp. 214-223, (2017) (PubMed).

Chen, Zhou, Yan, Ma, Zheng: "Hyperosmotic stress induces cisplatin sensitivity in ovarian cancer cells by stimulating aquaporin-5 expression." in: Experimental and therapeutic medicine, Vol. 10, Issue 6, pp. 2055-2062, (2015) (PubMed).

Target Details for Aquaporin 5

Target: Aquaporin 5 (AQP5)

Alternative Name: AQP5

Background:

Synonyms: Aquaporin-5;AQP-5;Aqp5;

Background: Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal Müller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date (AQPs 0-6), genes encoding the four most closely related aquaporins (AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.

Molecular Weight: 62 kDa