ATG7 antibody (C-Term)

Catalog No. ABIN3043718

The Rabbit Polyclonal anti-ATG7 antibody has been validated for WB. It is suitable to detect ATG7 in samples from Human, Mouse and Rat. There are 3+ publications available.

Quick Overview for ATG7 antibody (C-Term) (ABIN3043718)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG7 Antibody

Binding Specificity: AA 673-703, C-Term

Purpose: Anti-Apg7/ATG7 Antibody Picoband®

Sequence: HSFLEDLTGL TLLHQETQAA EIWDMSDDET I

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Apg7/ATG7 Antibody Picoband® (ABIN3043718). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae)". 2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Note: Erratum: Science 341: 457 only, 2013. 3. Tanida, I., Tanida-Miyake, T., Ueno, T., Kominami, E. The human homolog of Saccharomyces cerevisiae Apg7p is a protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J. Biol. Chem. 276: 1701-1706, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ATG7 antibody (ABIN3043718)

Shen, Li, Cai, Liu, Wang, Gan, Li, Wang, Sun, Deng, Liu, Li: "Erbin exerts a protective effect against inflammatory bowel disease by suppressing autophagic cell death." in: Oncotarget, Vol. 9, Issue 15, pp. 12035-12049, (2018) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Yang, Sheng, Yang, Li, Qin, Yu, Zhang: "Endocan silencing induces programmed cell death in hepatocarcinoma." in: Oncology letters, Vol. 14, Issue 5, pp. 5333-5339, (2017) (PubMed).

Target Details for ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7

Background:

Synonyms: Ubiquitin-like modifier-activating enzyme ATG7,ATG12-activating enzyme E1 ATG7,Autophagy-related protein 7,APG7-like,hAGP7,Ubiquitin-activating enzyme E1-like protein,ATG7,APG7L,

Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Background: Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

Sequence Similarities: Belongs to the aspartyl/asparaginyl beta-hydroxylase family.

Molecular Weight: 78 kDa

Gene ID: 10533

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy