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Connexin 43/GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 366-382) Primary Antibody

GJA1 Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (9)
Catalog No. ABIN3043758
$280.00
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target
    Connexin 43/GJA1 (GJA1)
    Binding Specificity
    • 22
    • 16
    • 15
    • 12
    • 8
    • 8
    • 8
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 366-382, C-Term
    Reactivity
    • 141
    • 97
    • 92
    • 35
    • 13
    • 12
    • 10
    • 10
    • 9
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 135
    • 10
    • 10
    Rabbit
    Clonality
    • 143
    • 11
    Polyclonal
    Conjugate
    • 89
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Connexin 43/GJA1 antibody is un-conjugated
    Application
    • 106
    • 58
    • 53
    • 29
    • 20
    • 18
    • 13
    • 13
    • 10
    • 10
    • 9
    • 6
    • 2
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein( GJA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Sequence
    RASSRASSRP RPDDLEI
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Gap junction alpha-1 protein( GJA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: gap junction protein, alpha 1, 43 kDa
    Protein Name: Gap junction alpha-1 protein
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(366-382aa RASSRASSRPRPDDLEI), identical to the related rat and mouse sequences.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Xu, Hu, Khan, Shi, Cong, Li, Dai, Dai: "Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43." in: Acta pharmacologica Sinica, Vol. 37, Issue 2, pp. 246-54, 2017 (PubMed).

    Zeng, Li, Li, Li, Xiao, Chu, Yang: "Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats." in: Bioengineered, Vol. 7, Issue 5, pp. 314-320, 2017 (PubMed).

    Ming, Zhou, Du, Fan, Pan, Wang, Fan, Jiang: "Identification of miR-200a as a novel suppressor of connexin 43 in breast cancer cells." in: Bioscience reports, Vol. 35, Issue 5, 2016 (PubMed).

    Ming, Zhou, Du, Fan, Pan, Wang, Fan, Jiang: "miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells." in: Bioscience reports, Vol. 35, Issue 6, 2016 (PubMed).

    Zhang, Gao, Dong, Wang, Yao, Zhang, Hu, Xu, Zuo, Wang, Zhou, Zhao, Peng: "The compound Chinese medicine "Kang Fu Ling" protects against high power microwave-induced myocardial injury." in: PLoS ONE, Vol. 9, Issue 7, pp. e101532, 2014 (PubMed).

    Li, Tang, Liang, Li, Wang, Song, Zheng, Xi, Zhang, Hescheler, Zhu: "Coculture of embryonic ventricular myocytes and mouse embryonic stem cell enhance intercellular signaling by upregulation of connexin43." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 1, pp. 53-63, 2013 (PubMed).

    Xing, Lv, Wang, Yan, Zhao, Cao: "Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats." in: Journal of biomedical science, Vol. 19, pp. 6, 2012 (PubMed).

    Zhang, Yu, Dai, Cheng, Zhang, Dai: "CPU86017-RS attenuate hypoxia-induced testicular dysfunction in mice by normalizing androgen biosynthesis genes and pro-inflammatory cytokines." in: Acta pharmacologica Sinica, Vol. 33, Issue 4, pp. 470-8, 2012 (PubMed).

    Peng, Dai, Ji, Dai: "The separate roles of endothelin receptors participate in remodeling of matrix metalloproteinase and connexin 43 of cardiac fibroblasts in maladaptive response to isoproterenol." in: European journal of pharmacology, Vol. 634, Issue 1-3, pp. 101-6, 2010 (PubMed).

  • Target
    Connexin 43/GJA1 (GJA1)
    Alternative Name
    GJA1 (GJA1 Antibody Abstract)
    Synonyms
    AVSD3, CX43, DFNB38, GJAL, HLHS1, HSS, ODDD, Cx43, AU042049, AW546267, Cnx43, Cx43alpha1, Gja-1, Npm1, connexin43, cx43a1, gja1, zfCx43.3, connexin 43, gja1-A, cx43, gjal, oddd, dfnb38, gap junction protein alpha 1, gap junction protein, alpha 1, connexin 43, gap junction protein alpha 1 L homeolog, CONNEXIN 43 protein, GJA1, Gja1, cx43, gja1.L, gja1, CONNEXIN 43
    Background
    Connexins 43(Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.

    Synonyms: Connexin 43 antibody|Connexin-43 antibody|Connexin43 antibody|Cx 43 antibody|CX43 antibody|CXA1_HUMAN antibody|DFNB38 antibody|Gap junction 43 kDa heart protein antibody|Gap junction alpha 1 protein antibody|Gap junction alpha-1 protein antibody|Gap junction protein alpha 1 43kD antibody|Gap junction protein alpha 1 43 kDa(connexin 43) antibody|Gap junction protein alpha 1 43 kDa antibody|Gap junction protein alpha like antibody|GJA 1 antibody|Gja1 antibody|GJAL antibody|HGNC:4282 antibody|HGNC:8112 antibody|Oculodentodigital dysplasia antibody|ODD antibody|ODDD antibody|ODOD antibody|SDTY 3 antibody|SDTY3 antibody|Syndactyly type III antibody
    UniProt
    P17302
    Pathways
    MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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