ABCC4 antibody (AA 1-370)

Catalog No. ABIN3043774

This Rabbit Polyclonal antibody specifically detects ABCC4 in WB, IHC. It exhibits reactivity toward Human, Mouse, Rat.

Quick Overview for ABCC4 antibody (AA 1-370) (ABIN3043774)

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ABCC4 Antibody

Binding Specificity: AA 1-370

Purpose: Anti-MRP4/ABCC4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MRP4/ABCC4 Antibody Picoband® (ABIN3043774). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MRP4 recombinant protein (Position: M1-P370).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human
1. Kool, M., de Haas, M., Scheffer, G. L., Scheper, R. J., van Eijk, M. J., Juijn, J. A., Baas, F., Borst, P. Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res. 57: 3537-3547, 1997. 2. Lee, K., Belinsky, M. G., Bell, D. W., Testa, J. R., Kruh, G. D. Isolation of MOAT-B, a widely expressed multidrug resistance-associated protein/canalicular multispecific organic anion transporter-related transporter. Cancer Res. 58: 2741-2747, 1998. 3. van de Ven, R., Scheffer, G. L., Reurs, A. W., Lindenberg, J. J., Oerlemans, R., Jansen, G., Gillet, J.-P., Glasgow, J. N., Pereboev, A., Curiel, D. T., Scheper, R. J., de Gruijl, T. D. A role for multidrug resistance protein 4 (MRP4, ABCC4) in human dendritic cell migration.Blood 112: 2353-2359, 2008.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ABCC4

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Alternative Name: ABCC4

Background:

Synonyms: Multidrug resistance-associated protein 4,ATP-binding cassette sub-family C member 4,MRP/cMOAT-related ABC transporter,Multi-specific organic anion transporter B,MOAT-B,ABCC4,MRP4,

Tissue Specificity: Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Background: ABCC4 (Atp-binding cassette, subfamily c, member 4) also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene. It belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. This gene is mapped to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of ABCC4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of ABCC4 activity or knockdown of ABCC4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. It has been found that ABCC4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.

Sequence Similarities: Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Molecular Weight: 47 kDa

Gene ID: 10257

UniProt: O15439