FE65 antibody (N-Term)

Catalog No. ABIN3043784

The Rabbit Polyclonal anti-FE65 antibody has been validated for WB and IHC. It is suitable to detect FE65 in samples from Mouse, Human and Rat.

Quick Overview for FE65 antibody (N-Term) (ABIN3043784)

Target: FE65 (APBB1) (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FE65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FE65 Antibody

Binding Specificity: AA 21-56, N-Term

Purpose: Anti-FE65/APBB1 Antibody Picoband®

Sequence: ALSLPLPLHA AHNQLLNAKL QATAVGPKDL RSAMGE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FE65/APBB1 Antibody Picoband® (ABIN3043784). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FE65, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Chen GB, et al. Association of amyloid precursor protein-binding protein, family B, member 1 with nicotine dependence in African and European American smokers. Hum Genet, 2008 Nov. 2. Cheung HN, et al. FE65 interacts with ADP-ribosylation factor 6 to promote neurite outgrowth. FASEB J, 2014 Jan. 3. Ward MW, et al. The amyloid precursor protein intracellular domain (AICD) disrupts actin dynamics and mitochondrial bioenergetics. J Neurochem, 2010 Apr. 

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FE65

Target: FE65 (APBB1) (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))

Alternative Name: APBB1

Background:

Synonyms: Amyloid beta A4 precursor protein-binding family B member 1,Protein Fe65,APBB1,FE65, RIR,

Tissue Specificity: Highly expressed in brain, strongly reduced in post-mortem elderly subjects with Alzheimer disease. .

Background: APBB1 is also known as RIR or FE65. The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 65 kDa

Gene ID: 322

UniProt: O00213

Pathways: Positive Regulation of Response to DNA Damage Stimulus