ATP5H antibody (AA 2-161)

Catalog No. ABIN3043795

This Rabbit Polyclonal antibody specifically detects ATP5H in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for ATP5H antibody (AA 2-161) (ABIN3043795)

Target: ATP5H (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATP5H Antibody

Binding Specificity: AA 2-161

Purpose: Anti-ATP5H Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ATP5H Antibody Picoband® (ABIN3043795). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section),2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
1. Boada M, et al. ATP5H/KCTD2 locus is associated with Alzheimer's disease risk. Mol Psychiatry, 2014 Jun. 2. Kim DW, et al. Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells. Cancer Sci, 2008 Oct.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ATP5H

Target: ATP5H (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D (ATP5H))

Alternative Name: ATP5H

Background:

Synonyms: ATP synthase subunit d, mitochondrial,ATPase subunit d,ATP5H,My032,

Tissue Specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20 % of very low density lipoproteins (VLDL) and 1-2 % of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Background: ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Sequence Similarities: Belongs to the apolipoprotein A1/A4/E family.

Molecular Weight: 22 kDa

Gene ID: 10476

UniProt: O75947

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process