CEBPB antibody (AA 1-200)

Catalog No. ABIN3043809

This anti-CEBPB antibody is a Rabbit Polyclonal antibody detecting CEBPB in WB, IHC, IF, ICC and FACS. Suitable for Human.

Quick Overview for CEBPB antibody (AA 1-200) (ABIN3043809)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-CEBPB Antibody

Binding Specificity: AA 1-200

Purpose: Anti-CEBP Beta/CEBPB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CEBP Beta/CEBPB Antibody Picoband® (ABIN3043809). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CEBP Beta recombinant protein (Position: M1-A200). Human CEBP Beta shares 61% amino acid (aa) sequence identity with both mouse and rat CEBP Beta.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Szpirer C, Riviere M, Cortese R, Nakamura T, Islam MQ, Levan G, Szpirer J (Jul 1992). "Chromosomal localization in man and rat of the genes encoding the liver-enriched transcription factors C/EBP, DBP, and HNF1/LFB-1 (CEBP, DBP, and transcription factor 1, TCF1, respectively) and of the hepatocyte growth factor/scatter factor gene (HGF)". Genomics 13 (2): 293-300. 2. Cao Z, Umek RM, McKnight SL (Oct 1991). "Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells". Genes Dev 5 (9): 1538-52. 3. Harries LW, Pilling LC, Hernandez LD, Bradley-Smith R, Henley W, Singleton AB, Guralnik JM, Bandinelli S, Ferrucci L, Melzer D (2012).

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: CEBPB

Background:

Synonyms: CCAAT/enhancer-binding protein beta ,C/EBP beta ,Liver activator protein,LAP,Liver-enriched inhibitory protein,LIP,Nuclear factor NF-IL6,Transcription factor 5,TCF-5,CEBPB ,TCF5,PP9092,

Tissue Specificity: Expressed at low levels in the lung, kidney and spleen.

Background: CCAAT/enhancer-binding protein beta, also known as is CEBP-beta, is a protein that in humans is encoded by the CEBPB gene. It mapped to 20q13.13. The protein encoded by this intronless gene is a bZIP transcription factor that can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. CEBP-beta is critical for normal macrophage functioning, an important immune cell sub-type. Observational work has shown that expression of CEBP-beta in blood leukocytes is positively associated with muscle strength in humans, emphasizing the importance of the immune system, and particularly macrophages, in the maintenance of muscle function.

Sequence Similarities: Belongs to the bZIP family. C/EBP subfamily.

Molecular Weight: 46 kDa

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation