HSPD1 antibody (AA 260-496)

Catalog No. ABIN3043854

Product Details anti-HSPD1 Antibody

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Binding Specificity: AA 260-496

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: heat shock 60 kDa protein 1 (chaperonin)
Protein Name: 60 kDa heat shock protein, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Hsp60 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-HSPD1 antibody (ABIN3043854)

Li, Nan, Zhai, Wang, Si, Chang: "Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 143-50, (2014) (PubMed).

Li, Zhai, Wang, Si, Wu, Chang: "Hsp60 in caudal fin regeneration from Paramisgurnus dabryanus: molecular cloning and expression characterization." in: Fish & shellfish immunology, Vol. 36, Issue 2, pp. 401-8, (2014) (PubMed).

Su, Baker, Christian, Naples, Tong, Zhang, Santha, Adeli: "Hepatic mitochondrial and ER stress induced by defective PPARα signaling in the pathogenesis of hepatic steatosis." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 11, pp. E1264-73, (2014) (PubMed).

Hager, Li, Pun, Liu, Hossain, Maguire, Naples, Baker, Magomedova, Tam, Adeli, Cummins, Connelly, Ng: "Lecithin:cholesterol acyltransferase deficiency protects against cholesterol-induced hepatic endoplasmic reticulum stress in mice." in: The Journal of biological chemistry, Vol. 287, Issue 24, pp. 20755-68, (2012) (PubMed).

Cui, Du, Lu, Qiang: "Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress." in: Journal of insect science (Online), Vol. 10, pp. 100, (2010) (PubMed).

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1 (HSPD1 Products)

Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.

Synonyms: 60 kDa chaperonin antibody|60 kDa heat shock protein, mitochondrial antibody|60 kDa antibody|cb863 antibody|CH60_HUMAN antibody|Chaperonin 60 antibody|Chaperonin antibody|Chaperonin, 60-KD antibody|CPN60 antibody|GROEL antibody|GroEL, E. coli, homolog of antibody|Heat shock 60kD protein 1 (chaperonin) antibody|heat shock 60 kDa protein 1 (chaperonin) antibody|Heat shock 60 kDa protein 1 antibody|Heat shock protein 1 (chaperonin) antibody|Heat shock protein 60 antibody|Heat shock protein 65 antibody|HLD4 antibody|Hsp 60 antibody|HSP 65 antibody|HSP-60 antibody|HSP60 antibody|HSP65 antibody|HSPD1 antibody|HuCHA60 antibody|Mitochondrial matrix protein P1 antibody|P60 lymphocyte protein antibody|Short heat shock protein 60 Hsp60s1 antibody|Spastic paraplegia 13 (autosomal dominant) antibody|SPG13 antibody

Gene ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity