HSPD1 antibody (AA 260-496)
-
- Target See all HSPD1 Antibodies
- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
-
Binding Specificity
- AA 260-496
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This HSPD1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Purpose
- Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
-
Rabbit IgG polyclonal antibody for 60 kDa heat shock protein, mitochondrial(HSPD1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: heat shock 60 kDa protein 1 (chaperonin)
Protein Name: 60 kDa heat shock protein, mitochondrial - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
- Isotype
- IgG
- Top Product
- Discover our top product HSPD1 Primary Antibody
-
-
- Application Notes
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Hsp60 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
-
Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 143-50, (2014) (PubMed).
: "Hsp60 in caudal fin regeneration from Paramisgurnus dabryanus: molecular cloning and expression characterization." in: Fish & shellfish immunology, Vol. 36, Issue 2, pp. 401-8, (2014) (PubMed).
: "Hepatic mitochondrial and ER stress induced by defective PPARα signaling in the pathogenesis of hepatic steatosis." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 11, pp. E1264-73, (2014) (PubMed).
: "Lecithin:cholesterol acyltransferase deficiency protects against cholesterol-induced hepatic endoplasmic reticulum stress in mice." in: The Journal of biological chemistry, Vol. 287, Issue 24, pp. 20755-68, (2012) (PubMed).
: "Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress." in: Journal of insect science (Online), Vol. 10, pp. 100, (2010) (PubMed).
: "
-
Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 143-50, (2014) (PubMed).
-
- Target
- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
- Alternative Name
- HSPD1 (HSPD1 Products)
- Background
-
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Synonyms: 60 kDa chaperonin antibody|60 kDa heat shock protein, mitochondrial antibody|60 kDa antibody|cb863 antibody|CH60_HUMAN antibody|Chaperonin 60 antibody|Chaperonin antibody|Chaperonin, 60-KD antibody|CPN60 antibody|GROEL antibody|GroEL, E. coli, homolog of antibody|Heat shock 60kD protein 1 (chaperonin) antibody|heat shock 60 kDa protein 1 (chaperonin) antibody|Heat shock 60 kDa protein 1 antibody|Heat shock protein 1 (chaperonin) antibody|Heat shock protein 60 antibody|Heat shock protein 65 antibody|HLD4 antibody|Hsp 60 antibody|HSP 65 antibody|HSP-60 antibody|HSP60 antibody|HSP65 antibody|HSPD1 antibody|HuCHA60 antibody|Mitochondrial matrix protein P1 antibody|P60 lymphocyte protein antibody|Short heat shock protein 60 Hsp60s1 antibody|Spastic paraplegia 13 (autosomal dominant) antibody|SPG13 antibody - Gene ID
- 3329
- UniProt
- P10809
- Pathways
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
-