IGF2R antibody (C-Term)

Catalog No. ABIN3044000

The Rabbit Polyclonal anti-IGF2R antibody has been validated for WB and FACS. It is suitable to detect IGF2R in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for IGF2R antibody (C-Term) (ABIN3044000)

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF2R antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-IGF2R Antibody

Binding Specificity: AA 2475-2491, C-Term

Purpose: Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband®

Sequence: TKLVSFHDDS DEDLLHI

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody (ABIN3044000). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Mannose 6 Phosphate Receptor(Cation independent), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Killian, J. K., Oka, Y., Jang, H.-S., Fu, X., Waterland, R. A., Sohda, T., Sakaguchi, S., Jirtle, R. L. Mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) variants in American and Japanese populations. Hum. Mutat. 18: 25-31, 2001. 2. Latos, P. A., Pauler, F. M., Koerner, M. V., Senergin, H. B., Hudson, Q. J., Stocsits, R. R., Allhoff, W., Stricker, S. H., Klement, R. M., Warczok, K. E., Aumayr, K., Pasierbek, P., Barlow, D. P. Airn transcriptional overlap, but not its IncRNA products, induces imprinted Igf2r silencing. Science 338: 1469-1472, 2012.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IGF2R antibody (ABIN3044000)

Liang, Hao, Zhang, Wu, Wang: "Insulin-like growth factors in endometrioid adenocarcinoma: correlation with clinico-pathological features and estrogen receptor expression." in: BMC cancer, Vol. 12, pp. 262, (2013) (PubMed).

Target Details for IGF2R

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Alternative Name: IGF2R

Background:

Synonyms: Cation-independent mannose-6-phosphate receptor,CI Man-6-P receptor,CI-MPR,M6PR,300 kDa mannose 6-phosphate receptor,MPR 300,Insulin-like growth factor 2 receptor,Insulin-like growth factor II receptor,IGF-II receptor,M6P/IGF2 receptor,M6P/IGF2R,CD222,IGF2R,MPRI,

Tissue Specificity: Highly expressed in liver, kidney, lung and skin. .

Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

Sequence Similarities: Belongs to the MRL1/IGF2R family.

Molecular Weight: 274 kDa

UniProt: P11717