CYP2U1 antibody (C-Term)

Catalog No. ABIN3044054

The Rabbit Polyclonal anti-CYP2U1 antibody has been validated for WB, IHC and ICC. It is suitable to detect CYP2U1 in samples from Human.

Quick Overview for CYP2U1 antibody (C-Term) (ABIN3044054)

Target: CYP2U1 (Cytochrome P450, Family 2, Subfamily U, Polypeptide 1 (CYP2U1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP2U1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CYP2U1 Antibody

Binding Specificity: AA 457-473, C-Term

Purpose: Anti-Cytochrome P450 2U1 CYP2U1 Antibody Picoband®

Sequence: EKPEDFYPNR FLDDQGQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cytochrome 2U1 CYP2U1 Antibody (ABIN3044054). Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP2U1.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Western blot, 0.1-0.5 μg/mL, Human
1. Chuang, S. S., Helvig, C., Taimi, M., Ramshaw, H. A., Collop, A. H., Amad, M., White, J. A., Petkovich, M., Jones, G., Korczak, B. CYP2U1, a novel human thymus- and brain-specific cytochrome P450, catalyzes omega- and (omega-1)-hydroxylation of fatty acids. J. Biol. Chem. 279: 6305-6314, 2004. 2. Tesson, C., Nawara, M., Salih, M. A. M., Rossignol, R., Zaki, M. S., Al Balwi, M., Schule, R., Mignot, C., Obre, E., Bouhouche, A., Santorelli, F. M., Durand, C. M., and 30 others. Alteration of fatty-acid-metabolizing enzymes affects mitochondrial form and function in hereditary spastic paraplegia. Am. J. Hum. Genet. 91: 1051-1064, 2012.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CYP2U1

Target: CYP2U1 (Cytochrome P450, Family 2, Subfamily U, Polypeptide 1 (CYP2U1))

Alternative Name: CYP2U1

Background:

Synonyms: Cytochrome P450 2U1,1.14.14.1,CYP2U1,

Tissue Specificity: Widely expressed with stronger expression in thymus, heart and cerebellum. .

Background: CYP2U1 (Cytochrome P450, Family 2, Subfamily U, Polypeptide 1), is a protein that in humans is encoded by the CYP2U1 gene. Members of the P450 enzyme family have roles in the tissue-specific conversion of substrates into locally active hormones, < a href="https://www.bosterbio.com/blog/post/detecting-vitamins-in-laboratories" style="color:#EA8D28">vitamins, and signaling molecules, including arachidonic acid derivatives known as eicosanoids. The International Radiation Hybrid Mapping Consortium mapped the CYP2U1 gene to chromosome 4.Chuang et al. (2004) stated that CYP2U1 maps to 4q25. Using recombination experiments in Sf9 insect cells, Chuang et al. (2004) found that CYP2U1 metabolized arachidonic acid, docosahexaenoic acid (DHA), and other long chain fatty acids. Chuang et al. (2004) suggested that CYP2U1 may play a role in brain and immune functions.

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molecular Weight: 22 kDa

UniProt: Q7Z449