EIF6 antibody (Middle Region)

Catalog No. ABIN3044059

This Rabbit Polyclonal antibody specifically detects EIF6 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EIF6 antibody (Middle Region) (ABIN3044059)

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF6 Antibody

Binding Specificity: AA 82-96, Middle Region

Purpose: Anti-integrin beta 4 binding protein/EIF6 Antibody Picoband®

Sequence: QHIRNSLPDT VQIRR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-integrin beta 4 binding protein/EIF6 Antibody (ABIN3044059). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EIF6, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Ceci, M., Gaviraghi, C., Gorrini, C., Sala, L. A., Offenhauser, N., Marchisio, P. C., Biffo, S. Release of eIF6 (p27-BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426: 579-584, 2003. 2. Gandin, V., Miluzio, A., Barbieri, A. M., Beugnet, A., Kiyokawa, H., Marchisio, P. C., Biffo, S. Eukaryotic initiation factor 6 is rate-limiting in translation, growth and transformation. Nature 455: 684-688, 2008. 3. Sanvito, F., Arrigo, G., Zuffardi, O., Agnelli, M., Marchisio, P. C., Biffo, S. Localization of p27 beta-4 binding protein gene (ITGB4BP) to human chromosome region 20q11.2. Genomics 52: 111-112, 1998.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EIF6

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Alternative Name: EIF6

Background:

Synonyms: Eukaryotic translation initiation factor 6 ,eIF-6 ,B (2)GCN homolog,B4 integrin interactor,CAB,p27 (BBP),EIF6 ,EIF3A, ITGB4BP ,OK/SW-cl.27,

Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .

Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Sequence Similarities: Belongs to the eIF-3 subunit B family.

Molecular Weight: 115 kDa

UniProt: P56537

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly