CD1d antibody (N-Term)

Catalog No. ABIN3044081

The Rabbit Polyclonal anti-CD1d antibody has been validated for WB, IHC. It is suitable to detect CD1d in samples from Human.

Quick Overview for CD1d antibody (N-Term) (ABIN3044081)

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD1d antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD1d Antibody

Binding Specificity: AA 76-92, N-Term

Purpose: Anti-CD1d Antibody Picoband®

Sequence: FSDQQWETLQ HIFRVYR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD1d Antibody (ABIN3044081). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD1d.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Jayawardena-Wolf, J., Benlagha, K., Chiu, Y.-H., Mehr, R., Bendelac, A. CD1d endosomal trafficking is independently regulated by an intrinsic CD1d-encoded tyrosine motif and by the invariant chain.Immunity 15: 897-908, 2001.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD1d

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Alternative Name: CD1D

Background:

Synonyms: Antigen-presenting glycoprotein CD1d;R3G1;CD1d;CD1D;

Tissue Specificity: Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

Background: CD1D (CD1d molecule) also known as R3G1, Antigen-presenting glycoprotein CD1d, R3G1, CD1D, is the sole group-2 member of the CD1 family of major histocompatibility (MHC)-like glycoproteins. Expressed on the surface of various human antigen-presenting cells. They are related to the class I MHC molecules, and are involved in the presentation of lipid antigens to T cells. It is the only member of the group 2 CD1 molecules. CD1d presented lipid antigens activate a special class of T cells, known as Natural Killer T (NKT) cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and IL-4 production. Jayawardena-Wolf et al. described 2 different pathways of Cd1d trafficking to endosomal compartments in mouse cells. A tyrosine-based motif governs recycling between the plasma membrane and the endosome, while Cd1d associates, like major histocompatibility complex class II antigens, with the invariant chain, or II, in the endoplasmic reticulum. Both pathways enhance antigen presentation to Cd1d-restricted natural killer T cells.

Sequence Similarities: Belongs to the peptidase C14A family.

Molecular Weight: 31 kDa

UniProt: P15813

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process