KLF3 antibody (N-Term)

Catalog No. ABIN3044105

The Rabbit Polyclonal anti-KLF3 antibody has been validated for WB. It is suitable to detect KLF3 in samples from Human, Mouse and Rat.

Quick Overview for KLF3 antibody (N-Term) (ABIN3044105)

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KLF3 Antibody

Binding Specificity: AA 20-34, N-Term

Purpose: Anti-Krueppel-like factor 3 KLF3 Antibody Picoband®

Sequence: SYPSNYMESM KPNKY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Krueppel-like factor 3 KLF3 Antibody (ABIN3044105). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Suske, G., Bruford, E., Philipsen, S.Mammalian SP/KLF transcription factors: bring in the family.Genomics 85: 551-556, 2005. 2. Turner, Jeremy, Nicholas Hannah, Bishop David, Matthews Jacqueline M, Crossley Merlin "The LIM protein FHL3 binds basic Krüppel-like factor/Krüppel-like factor 3 and its co-repressor C-terminal-binding protein 2". J. Biol. Chem. (United States) 278 (15): 12786-95. 2003 3. Wang MJ, Qu XH, Wang LS, et al.. "[cDNA cloning, subcellular localization and tissue expression of a new human Krüppel-like transcription factor: human basic Krüppel-like factor (hBKLF)]". Yi Chuan Xue Bao 30 (1): 1-9. 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KLF3

Target: KLF3 (Kruppel-Like Factor 3 (Basic) (KLF3))

Alternative Name: KLF3

Background:

Synonyms: Krueppel-like factor 3;Basic krueppel-like factor;CACCC-box-binding protein BKLF;TEF-2;KLF3;BKLF;

Background: KLF3 (Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF (Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein (CtBP). Suske et al. (2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al. (2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.

Sequence Similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.

Molecular Weight: 18 kDa

UniProt: P57682