HDAC3 antibody (C-Term)

Catalog No. ABIN3044210

The Rabbit Polyclonal anti-HDAC3 antibody has been validated for WB, IHC, IF, FACS, ICC and IHC (fro). It is suitable to detect HDAC3 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for HDAC3 antibody (C-Term) (ABIN3044210)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HDAC3 Antibody

Binding Specificity: AA 411-428, C-Term

Purpose: Anti-Histone deacetylase 3 HDAC3 Antibody Picoband®

Sequence: NEFYDGDHDN DKESDVEI

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Histone deacetylase 3 HDAC3 Antibody (ABIN3044210). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC3, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Alenghat, T., Meyers, K., Mullican, S. E., Leitner, K., Adeniji-Adele, A., Avila, J., Bucan, M., Ahima, R. S., Kaestner, K. H., Lazar, M. A. Nuclear receptor corepressor and histone deacetylase 3 govern circadian metabolic physiology. Nature 456: 997-1000, 2008. 2. Chen, L., Fischle, W., Verdin, E., Greene, W. C. Duration of nuclear NF-kappa-B action regulated by reversible acetylation. Science 293: 1653-1657, 2001. 3. Dangond F, Hafler DA, Tong JK, Randall J, Kojima R, Utku N, Gullans SR (Mar 1998). "Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cells". Biochem Biophys Res Commun 242 (3): 648-52. doi:10.1006/bbrc.1997.8033.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-HDAC3 antibody (ABIN3044210)

Zhao, Chen, Li, Zhang, Li, Das, Bean, Kong, Zhu, Gao: "Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation." in: BMC neuroscience, Vol. 10, pp. 113, (2009) (PubMed).

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background:

Synonyms: Histone deacetylase 3,HD3,3.5.1.98,RPD3-2,SMAP45,HDAC3,

Tissue Specificity: Widely expressed.

Background: HDAC3 (HISTONE DEACETYLASE 3) is a member of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC3 gene. The HDAC3 gene is mapped to 5q31.3. HDAC3 has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. And this gene is regarded as a potential tumor suppressor gene. HDAC3 has an open reading frame of 428 amino acids and shares 53 % amino acid identity with HDAC1 and 52 % with HDAC2. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4. HDAC3 recruitment to the genome displays a circadian rhythm in mouse liver.

Sequence Similarities: Belongs to the histone deacetylase family. HD type 1 subfamily.

Molecular Weight: 45 kDa

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development