p65 antibody (Nuclear Factor-KB P65) (AA 143-158)

Details for Product anti-NFkBP65 Antibody No. ABIN3044253
Antigen
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Alternatives
anti-Human p65 antibody for Immunoprecipitation
Epitope
AA 143-158, Middle Region
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Reactivity
Human, Rat (Rattus)
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Host
Rabbit
1143
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Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Purpose Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human NF-kB p65(143-158aa VPIEEQRGDYDLNAVR), identical to the related rat and mouse sequences.
Sequence VPIEEQRGDY DLNAVR
Isotype IgG
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: v-rel reticuloendotheliosis viral oncogene homolog A(avian)
Protein Name: Transcription factor p65
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others p65 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name RELA (NFkBP65 Antibody Abstract)
Background RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.

Synonyms: Avian reticuloendotheliosis viral(v rel) oncogene homolog A antibody|MGC131774 antibody|NF kappa B p65delta3 antibody|NFKB 3 antibody|NFKB3 antibody|Nuclear Factor NF Kappa B p65 Subunit antibody|Nuclear factor NF-kappa-B p65 subunit antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody|Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody|OTTHUMP00000233473 antibody|OTTHUMP00000233474 antibody|OTTHUMP00000233475 antibody|OTTHUMP00000233476 antibody|OTTHUMP00000233900 antibody|p65 antibody|p65 NF kappaB antibody|p65 NFkB antibody|RELA antibody|TF65_HUMAN antibody|Transcription Factor p65 antibody|v rel avian reticuloendotheliosis viral oncogene homolog A(nuclear factor of kappa light polypeptide gene enhancer in B cells 3(p65)) antibody|V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody|v rel reticuloendotheliosis viral oncogene homolog A(avian) antibody|V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody|v-rel reticuloendotheliosis viral oncogene homolog A antibody
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 143-158), (Middle Region) antibody (ABIN3044253) Anti-NF-kB p65 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 143-158), (Middle Region) antibody (ABIN3044253) Anti-NF-kB p65 antibody, Western blotting Lane 1: Rat Testis Tissue Lysate Lane 2: HE...
Product cited in: Hu, Fan, Li, Guan, Qu, Pei, Liu: "Bortezomib protects against dextran sulfate sodium‑induced ulcerative colitis in mice." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 4093-4099, 2018 (PubMed).

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