Annexin V antibody (Middle Region)

Catalog No. ABIN3044315

The Rabbit Polyclonal anti-Annexin V antibody has been validated for WB, IHC and ICC. It is suitable to detect Annexin V in samples from Human, Mouse and Rat. There are 7+ publications available.

Quick Overview for Annexin V antibody (Middle Region) (ABIN3044315)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin V antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Annexin V Antibody

Binding Specificity: AA 88-102, Middle Region

Purpose: Anti-Annexin V/ANXA5 Antibody Picoband®

Sequence: SRLYDAYELK HALKG

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Annexin V/ANXA5 Antibody (ABIN3044315). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Annexin V, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Kaplan, R., Jaye, M., Burgess, W. H., Schlaepfer, D. D., Haigler, H. T. : Cloning and expression of cDNA for human endonexin II, a Ca(2+) and phospholipid binding protein. J. Biol. Chem. 263 2. Rodriguez-Garcia, M. I., Kozak, C. A., Morgan, R. O., Fernandez, M. P. : Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution. Genomics 31: 151-157, 1996. 3. Grundmann, U., Abel, K.-J., Bohn, H., Lobermann, H., Lottspeich, F., Kupper, H. : Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family. Proc. Nat. Acad. Sci. 85: 3708-3712, 1988.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Annexin V antibody (ABIN3044315)

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Sun, Kang, Yao, Chen, Sun, An, Jiang, Wang, Hu: "Dehydrocostus lactone suppressed the proliferation, migration, and invasion of colorectal carcinoma through the downregulation of eIF4E expression." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 641-8, (2015) (PubMed).

Ji, Li, Wei, Kong, Jin, Chen, Li, Deng: "Effect of celastrol on growth inhibition of prostate cancer cells through the regulation of hERG channel in vitro." in: BioMed research international, Vol. 2015, pp. 308475, (2015) (PubMed).

Xu, Bi, Li, Fang, Zhou, Li, Chi, Wan, Shen: "Morphological and proteomic analyses reveal that unsaturated guluronate oligosaccharide modulates multiple functional pathways in murine macrophage RAW264.7 cells." in: Marine drugs, Vol. 13, Issue 4, pp. 1798-818, (2015) (PubMed).

Hu, Dong, Shi, Deng, Li, Lu: "Evaluation of a novel tetra-functional branched poly(ethylene glycol) crosslinker for manufacture of crosslinked, decellularized, porcine aortic valve leaflets." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 102, Issue 2, pp. 322-36, (2014) (PubMed).

Xiong, Ma, Zhang, Shao, Zhang, Zhou: "Apoptosis induced by ?,?-dimethylacrylshikonin is associated with Bcl-2 and NF-?B in human breast carcinoma MCF-7 cells." in: Oncology letters, Vol. 6, Issue 6, pp. 1789-1793, (2013) (PubMed).

Jin, Liu, Wang, Xu, Yang, Lu, Sun, Hu: "Protective effects of [Gly14]-Humanin on beta-amyloid-induced PC12 cell death by preventing mitochondrial dysfunction." in: Neurochemistry international, Vol. 56, Issue 3, pp. 417-23, (2010) (PubMed).

Target Details for Annexin V

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: ANXA5

Background:

Synonyms: Annexin A5,Anchorin CII,Annexin V,Annexin-5,Calphobindin I,CBP-I,Endonexin II,Lipocortin V,Placental anticoagulant protein 4,PP4,Placental anticoagulant protein I,PAP-I,Thromboplastin inhibitor,Vascular anticoagulant-alpha,VAC-alpha,ANXA5,ANX5, ENX2, PP4,

Background: Annexin V also know as endonnexin II (ENX2),or placental protein 4 (PP4). Endonexin II is a member of the family of Ca (2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an in inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Sequence Similarities: Belongs to the annexin family.

Molecular Weight: 36 kDa

UniProt: P08758

Pathways: Apoptosis