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GAD65 antibody (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)) (AA 21-37) Primary Antibody

GAD2 Reactivity: Human, Rat IHC (p), WB Host: Rabbit Polyclonal
Pubmed (2)
Catalog No. ABIN3044396
$280.00
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    AA 21-37, N-Term
    Reactivity
    Human, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This GAD65 antibody is un-conjugated
    Application
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Sequence
    ENPGTARAWC QVAQKFT
    Cross-Reactivity (Details)
    Predicted Cross Reactivity: mouse
    No cross reactivity with other proteins.
    Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
    Characteristics
    Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: glutamate decarboxylase 2(pancreatic islets and brain, 65 kDa)
    Protein Name: Glutamate decarboxylase 2
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.
    Isotype
    IgG
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Tian, Ma, Shi, Lou, Liu, Li: "Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, 2015 (PubMed).

    Shi, Ma, Zheng, Ruan, Liu, Tian, Zhang, He, Li: "Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum." in: Toxicology and industrial health, Vol. 28, Issue 1, pp. 10-20, 2012 (PubMed).

  • Target
    Alternative Name
    GAD2 (GAD2 Antibody Abstract)
    Synonyms
    GAD65, gad65, F12P19.12, F12P19_12, GLUTAMATE DECARBOXYLASE 2, glutamate decarboxylase 2, zgc:112198, 6330404F12Rik, GAD(65), Gad-2, glutamate decarboxylase 2, glutamate decarboxylase 1, glutamic acid decarboxylase 2, GAD2, PTRG_06148, LOC9324534, Gad2, GAD1, gad2
    Background
    Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

    Synonyms: 65 kDa glutamic acid decarboxylase antibody|DCE 2 antibody|DCE2 antibody|DCE2_HUMAN antibody|GAD 2 antibody|GAD 65 antibody|GAD-2 antibody|GAD-65 antibody|Gad2 antibody|Glutamate decarboxylase 2(pancreas) antibody|Glutamate Decarboxylase 2(pancreatic islets and brain 65 kDa) antibody|Glutamate decarboxylase 2 antibody|Glutamate Decarboxylase 65 antibody|Glutamate decarboxylase 65 kDa isoform antibody|Glutamic Acid Decarboxylase 2 antibody|Glutamic Acid Decarboxylase 65 antibody|MGC161605 antibody|MGC161607 antibody
    UniProt
    Q05329
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