GAD65 antibody (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)) (Internal Region)

Catalog No. ABIN185388

Product Details anti-GAD65 Antibody

Target:

GAD65 (GAD2)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: GAD2 / GAD65

Blocking Peptide: Blocking peptide for this product available: ABIN369972

Sequence: TLEDNEERMS RLSK

Specificity: This antibody is expected to recognise both reported variants.

Predicted Reactivity: Human, Mouse, Rat, Dog

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-TLEDNEERMSRLSK

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 65 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4 kDa according to NP_000809.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GAD65

Target:

GAD65 (GAD2)

Alternative Name: GAD2 (GAD2 Antibody Abstract)

Background: GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2

Gene ID: 2572

NCBI Accession: NP_000809, NP_001127838