MTA1 antibody (C-Term)

Catalog No. ABIN3044412

Product Details anti-MTA1 Antibody

Target: MTA1 (Metastasis Associated 1 (MTA1))

Binding Specificity: AA 676-696, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Anti-MTA1 Antibody Picoband®

Sequence: ETKRAARRPY KPIALRQSQA L

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MTA1 Antibody (ABIN3044412). Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MTA1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Cui, Q., Takiguchi, S., Matsusue, K., Toh, Y., Yoshida, M. A.Assignment of the human metastasis-associated gene 1 (MTA1) to human chromosome band 14q32.3 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 93: 139-140, 2001. 2. Reddy, S. D. N., Pakala, S. B., Ohshiro, K., Rayala, S. K., Kumar, R.MicroRNA-661, a c/EBP-alpha target, inhibits metastatic tumor antigen 1 and regulates its functions.Cancer Res. 69: 5639-5642, 2009. 3. Zhang, X., DeSalle, L. M., Patel, J. H., Capobianco, A. J., Yu, D., Thomas-Tikhonenko, A., McMahon, S. B.Metastasis-associated protein 1 (MTA1) is an essential downstream effector of the c-MYC oncoprotein.Proc. Nat. Acad. Sci. 102: 13968-13973, 2005.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MTA1

Target: MTA1 (Metastasis Associated 1 (MTA1))

Alternative Name: MTA1 (MTA1 Products)

Background:

Synonyms: Metastasis-associated protein MTA1,MTA1,

Tissue Specificity: Widely expressed. High expression in brain, liver, kidney, and cardiac muscle, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. Up-regulated in a wide variety of cancers including breast, liver, ovarian, and colorectal cancer and its expression levels are closely correlated with tumor aggressiveness and metastasis. .

Background: Metastasis-associated protein MTA1 is a protein that in humans is encoded by the MTA1 gene. This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues.By fluorescence in situ hybridization, mapped the MTA1gene to chromosome 14q32.3. MTA1 is a component of the chromatin remodeling complex that influences gene transcription by modulating target gene chromatin. MTA1 is widely upregulated in many carcinomas.

Sequence Similarities: Contains 1 BAH domain.

Molecular Weight: 80 kDa

UniProt: Q13330

Pathways: Chromatin Binding