EPHA1 antibody (C-Term)

Catalog No. ABIN3044443

Product Details anti-EPHA1 Antibody

Target: EPHA1 (Ephrin Type A Receptor 1 (EPHA1))

Binding Specificity: AA 963-976, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Ephrin type-A receptor 1(EPHA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: HQKRILCSIQ GFKD

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Ephrin type-A receptor 1(EPHA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: EPH receptor A1
Protein Name: Ephrin type-A receptor 1(hEpha1)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A1(963-976aa HQKRILCSIQGFKD), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EPHA1

Target: EPHA1 (Ephrin Type A Receptor 1 (EPHA1))

Alternative Name: EPHA1 (EPHA1 Products)

Synonyms: EPH antibody, EPHT antibody, EPHT1 antibody, 5730453L17Rik antibody, AL033318 antibody, Eph antibody, Esk antibody, EPHA9 antibody, EPH receptor A1 antibody, Eph receptor A1 antibody, EPHA1 antibody, Epha1 antibody

Background: EPHA1, EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The EPHA1 gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.

Synonyms: EPH antibody|Eph tyrosine kinase 1 antibody|EphA1 antibody|Ephrin receptor Eph A1 antibody|Ephrin type A receptor 1 antibody|EPHT 1 antibody|EPHT antibody|Erythropoietin producing hepatoma amplified sequence antibody|Esk antibody|MGC163163 antibody|Oncogene EPH antibody|Soluble EPHA1 variant 1 antibody|Soluble EPHA1 variant 2 antibody|Tyrosine protein kinase receptor EPH antibody

UniProt: P21709