Ceruloplasmin antibody (AA 20-259)

Catalog No. ABIN3044507

Product Details anti-Ceruloplasmin Antibody

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Binding Specificity: AA 20-259

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ceruloplasmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Ceruloplasmin/CP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Ceruloplasmin(CP) detection. Tested with WB, IHC-P in Human.
Gene Name: ceruloplasmin (ferroxidase)
Protein Name: Ceruloplasmin

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Ceruloplasmin recombinant protein (Position: K20-M259). Human Ceruloplasmin shares 80.8% and 79.6% amino acid (aa) sequence identity with mouse and rat Ceruloplasmin, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Koschinsky ML, Funk WD, van Oost BA, MacGillivray RT (Jul 1986)."Complete cDNA sequence of human preceruloplasmin". Proceedings of the National Academy of Sciences of the United States of America 83 (14): 5086-90. 2. Royle NJ, Irwin DM, Koschinsky ML, MacGillivray RT, Hamerton JL (May 1987). "Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively". Somatic Cell and Molecular Genetics 13(3): 285-92. 3. Takahashi N, Ortel TL, Putnam FW (Jan 1984). "Single-chain structure of human ceruloplasmin: the complete amino acid sequence of the whole molecule". Proceedings of the National Academy of Sciences of the United States of America 81 (2): 390-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Ceruloplasmin

Target: Ceruloplasmin (CP) (Ceruloplasmin (Ferroxidase) (CP))

Alternative Name: CP (CP Products)

Background:

Synonyms: Ceruloplasmin;1.16.3.1;Ferroxidase;CP;

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: Ceruloplasmin  (or caeruloplasmin) is a ferroxidase enzyme that in humans is encoded by the CP gene. It is mapped to 3q23-q25. The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe (II)transferrin to Fe (III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.

Molecular Weight: 122 kDa

Gene ID: 1356

UniProt: P00450

Pathways: Transition Metal Ion Homeostasis