CXCL2 antibody (AA 32-100)

Catalog No. ABIN3044513

The Rabbit Polyclonal anti-CXCL2 antibody has been validated for WB and ELISA. It is suitable to detect CXCL2 in samples from Rat and Mouse.

Quick Overview for CXCL2 antibody (AA 32-100) (ABIN3044513)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CXCL2 Antibody

Binding Specificity: AA 32-100

Purpose: Anti-CXCL2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CXCL2 Antibody Picoband® (ABIN3044513). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat CXCL2 recombinant protein (Position: S32-N100). Rat CXCL2 shares 62.3% and 88.4% amino acid (aa) sequence identity with huamn and mouse CXCL2, respectively.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Iida N., Grotendorst G.R. Cloning and sequencing of a new gro transcript from activated human monocytes: expression in leukocytes and wound tissue. Mol. Cell. Biol. 10:5596-5599, 1990. 2. Pelus LM, Fukuda S. Peripheral blood stem cell mobilization: the CXCR2 ligand GRObeta rapidly mobilizes hematopoietic stem cells with enhanced engraftment properties. Exp Hematol. 2006 Aug,34(8):1010-20. 3. Wolpe, S. D., Sherry, B., Juers, D., Davatelis, G., Yurt, R. W., Cerami, A. Identification and characterization of macrophage inflammatory protein 2. Proc. Nat. Acad. Sci. 86: 612-616, 1989.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: Cxcl2

Background:

Synonyms: C-X-C motif chemokine 2;Cytokine-induced neutrophil chemoattractant 3;CINC-3;Macrophage inflammatory protein 2;MIP2;Cxcl2;Cinc3, Mip-2, Mip2, Scyb2;

Tissue Specificity: At least expressed in the lung and trachea.

Background: Chemokine (C-X-C motif) ligand 2  (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha  (MIP2-alpha), Growth-regulated protein beta  (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1. This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines. CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.

Molecular Weight: 11 kDa

Gene ID: 114105

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin