Prokineticin 1 antibody (AA 20-105)

Catalog No. ABIN3044537

There is 1 publication for this product available. The Rabbit Polyclonal anti-Prokineticin 1 antibody is suitable to detect Prokineticin 1 in samples from Mouse. It has been validated for WB.

Quick Overview for Prokineticin 1 antibody (AA 20-105) (ABIN3044537)

Target: Prokineticin 1 (Prok1)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prokineticin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Prokineticin 1 Antibody

Binding Specificity: AA 20-105

Purpose: Anti-Prokineticin 1/PROK1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Prokineticin 1/PROK1 Antibody (ABIN3044537). Tested in WB applications. This antibody reacts with Mouse. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Prokineticin 1 recombinant protein (Position: A20-F105). Mouse Prokineticin 1 shares 88.4% and 94.2% amino acid (aa) sequence identity with human and rat Prokineticin 1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Prokineticin 1 antibody (ABIN3044537)

Yu, Chen, Jiang: "Administration of pigment epithelium-derived factor delivered by adeno-associated virus inhibits blood-retinal barrier breakdown in diabetic rats." in: Molecular vision, Vol. 16, pp. 2384-94, (2011) (PubMed).

Target Details for Prokineticin 1

Target: Prokineticin 1 (Prok1)

Alternative Name: PROK1

Background:

Synonyms: Prokineticin-1 ,Endocrine-gland-derived vascular endothelial growth factor ,EG-VEGF ,Prok1 ,Pk1 ,

Tissue Specificity: Highly expressed in liver and ovary and weakly expressed in testis and placenta. Expressed in mucosa and mesenchyme of embryonic gut during enteric nervous system development (at protein level). Predominantly expressed in kidney and liver. Also expressed in lung, ovary, placenta and testis. In fetal liver, is restricted to and highly expressed in hepatocytes. In adult kidney, expression is restricted to the endothelial tubule cells. In placenta, expressed throughout gestation. .

Background: Prokineticin-1 is also known as EG-VEGF. The International Radiation Hybrid Mapping Consortium mapped the PROK1 gene to chromosome 1. The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.

Molecular Weight: 12 kDa

Gene ID: 246691

UniProt: Q14A28