RAB9A antibody (C-Term)

Catalog No. ABIN3044539

This Rabbit Polyclonal antibody specifically detects RAB9A in WB, IF, ICC, FACS and IP. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for RAB9A antibody (C-Term) (ABIN3044539)

Target: RAB9A (RAB9A, Member RAS Oncogene Family (RAB9A))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB9A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-RAB9A Antibody

Binding Specificity: AA 156-193, C-Term

Purpose: Anti-Rab9/RAB9A Antibody Picoband®

Sequence: KDATNVAAAF EEAVRRVLAT EDRSDHLIQT DTVNLHRK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Rab9/RAB9A Antibody Picoband® (ABIN3044539). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Carroll, K. S., Hanna, J., Simon, I., Krise, J., Barbero, P., Pfeffer, S. R. Role of Rab9 GTPase in facilitating receptor recruitment by TIP47. Science 292: 1373-1376, 2001. 2. Davies, J. P., Cotter, P. D., Ioannou, Y. A. Cloning and mapping of human Rab7 and Rab9 cDNA sequences and identification of a Rab9 pseudogene. Genomics 41: 131-134, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB9A

Target: RAB9A (RAB9A, Member RAS Oncogene Family (RAB9A))

Alternative Name: RAB9A

Background:

Synonyms: Ras-related protein Rab-9A,RAB9A,RAB9,

Tissue Specificity: Widely expressed, high expression found in skeletal muscle. .

Background: Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is ly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What's more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.

Molecular Weight: 23 kDa

Gene ID: 9367

UniProt: P51151