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MHC, Class I antibody (FITC)

This Mouse Monoclonal antibody specifically detects MHC, Class I in FACS and IP. It exhibits reactivity toward Mouse, Human, Rat, Cow, Guinea Pig, Pig, Sheep and Hamster.
Catalog No. ABIN304990

Quick Overview for MHC, Class I antibody (FITC) (ABIN304990)

Target

See all MHC, Class I Antibodies
MHC, Class I

Reactivity

  • 71
  • 35
  • 13
  • 10
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 1
Mouse, Human, Rat, Cow, Guinea Pig, Pig, Sheep, Hamster

Host

  • 55
  • 47
  • 7
  • 1
Mouse

Clonality

  • 71
  • 36
  • 3
Monoclonal

Conjugate

  • 37
  • 14
  • 10
  • 8
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MHC, Class I antibody is conjugated to FITC

Application

  • 66
  • 53
  • 33
  • 23
  • 19
  • 15
  • 14
  • 13
  • 12
  • 11
  • 7
  • 5
  • 5
  • 3
Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone

2G5
  • Specificity

    Recognizes a monomorphic epitope on the mouse MHC class I antigen. The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this may recognize a polymorphic epitope of canine MHC class 1.

    Purification

    Affinity purified

    Immunogen

    Purified H-2K b and H-2D b MHC-I molecules.

    Isotype

    IgG2b
  • Application Notes

    Approved: Flo (1:1 - 1:5), IP

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    MHC, Class I

    Alternative Name

    MHC Class I
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