TJP2 antibody (C-Term)

Catalog No. ABIN305886

This anti-TJP2 antibody is a Rabbit Polyclonal antibody detecting TJP2 in WB, IHC, IF and IHC (p). Suitable for Human, Mouse, Rat and Dog.

Quick Overview for TJP2 antibody (C-Term) (ABIN305886)

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TJP2 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminal region of human or canine ZO-2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF (2.5 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunofluorescence: 2.5 μg/mL. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays. Positive control: Western Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.

Target Details for TJP2

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Alternative Name: TJP2 / ZO2 / ZO-2

Background: Name/Gene ID: TJP2
Family: Occludin

Synonyms: TJP2, C9DUPq21.11, DUP9q21.11, DFNA51, ZO-2, Zona occludens 2, Zonula occludens protein 2, Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104, Zona occludens protein 2

Gene ID: 9414

UniProt: Q9UDY2