CD31 antibody (PE)

Catalog No. ABIN306086

The Mouse Monoclonal anti-CD31 antibody has been validated for FACS, WB, IHC, ELISA and IHC (fro). It is suitable to detect CD31 in samples from Rat.

Quick Overview for CD31 antibody (PE) (ABIN306086)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD31 Antibody (PE)

Specificity: Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid,  pH 7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.

Purification: Protein G purified

Immunogen: Activated, Lewis rat derived microglial cells.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, WB

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mL sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA, 5 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Because Product is photosensitive, store in a dark location.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C. Do not freeze. Because product is photosensitive, store in a dark location.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CAM-1 / CD31

Background: Name/Gene ID: PECAM1

Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA

Gene ID: 5175

Pathways: Regulation of Actin Filament Polymerization