Hexokinase antibody (AA 1-917)

Catalog No. ABIN306422

The Mouse Monoclonal anti-Hexokinase antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect Hexokinase in samples from Human.

Quick Overview for Hexokinase antibody (AA 1-917) (ABIN306422)

Target: Hexokinase

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hexokinase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 4D7

Product Details anti-Hexokinase Antibody

Binding Specificity: AA 1-917

Purification: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography

Immunogen: Recombinant human Hexokinase1 (1-917aa) purified from E. coli

Isotype: IgG2a kappa

Application Details

Application Notes: Recommended dilution 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In Phosphate-Buffered Saline (pH7.4) with 0.1% Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details for Hexokinase

Target: Hexokinase

Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.

NCBI Accession: NP_000179