BUB1B antibody (AA 10-190)

Catalog No. ABIN306787

This Sheep Polyclonal antibody specifically detects BUB1B in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for BUB1B antibody (AA 10-190) (ABIN306787)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BUB1B Antibody

Binding Specificity: AA 10-190

Cross-Reactivity: Mouse

Characteristics: Mitotic checkpoint kinase MAD3L,Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

Purification: Ammonium Sulfate Precipitation

Immunogen: Recombinant protein corresponding to amino acids 10-190 of the mouse Bub1b protein.

Isotype: IgG

Application Details

Application Notes: Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis (1-10 μg/mL). Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: Bub1 beta

UniProt: Q9Z1S0