HSP27 antibody

Catalog No. ABIN306830

The Mouse Monoclonal anti-HSP27 antibody has been validated for WB, ICC, ELISA, IP, EIA, IHC (fro) and IHC (p). It is suitable to detect HSP27 in samples from Human.

Quick Overview for HSP27 antibody (ABIN306830)

Target: HSP27 (Heat Shock Protein 27 (HSP27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: G3-1

Product Details anti-HSP27 Antibody

Cross-Reactivity: Human

Characteristics: HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosphilia HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with partially purified HSP27 derived from MCF-7 cytosol and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: This antibody can be used for immunohistochemistry and immunocytochemistry on frozen and paraffin-embedded tissue sections as well as for ELISA, immunoprecipitation and Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for HSP27

Target: HSP27 (Heat Shock Protein 27 (HSP27))

Alternative Name: Heat Shock Protein 27

UniProt: P04792

Pathways: VEGF Signaling