SMC1A antibody (AA 402-894)

Catalog No. ABIN306835

This Mouse Monoclonal antibody specifically detects SMC1A in WB and IF. It exhibits reactivity toward Human.

Quick Overview for SMC1A antibody (AA 402-894) (ABIN306835)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: C2M

Product Details anti-SMC1A Antibody

Binding Specificity: AA 402-894

Cross-Reactivity: Human

Characteristics: Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus (2) protein in vivo.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 402-894 of human SMC1 and mouse myeloma cells.

Isotype: IgG1, kappa

Application Details

Application Notes: This antibody can be used for Western blotting (1-5 μg/mL) and immunofluorescence (5-10 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC-1

UniProt: Q14683

Pathways: Stem Cell Maintenance