Enolase antibody
This Mouse Monoclonal antibody specifically detects Enolase in ELISA, WB and IHC. It exhibits reactivity toward Human.
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Catalog No. ABIN307126
Quick Overview for Enolase antibody (ABIN307126)
Target
See all Enolase Antibodies
Enolase
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Conjugate
This Enolase antibody is un-conjugated
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Clone
NSE-P1
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Cross-Reactivity
- Chicken, Human, Mouse
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Characteristics
- ENO2, Enolase 2, (gamma, neuronal), 2-Phospho-D-Glycerate Hydrolase,Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, Enolase, Enolase and Enolase. Enolase expression has been detected on most tissues, whereas Enolase is expressed predominantly in muscle tissue and Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.
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Purification
- Protein A/G Chromatography
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Immunogen
- Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human NSE protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.
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Isotype
- IgG1
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anti-Enolase (C-Term) antibody
Reactivity: Human, Mouse, Rat ELISA, WB, IF, IHC Host: Rabbit Polyclonal unconjugated
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Application Notes
- Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.
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Restrictions
- For Research Use only
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Buffer
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- Enolase
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UniProt
- P09104
Target
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