VDAC1 antibody

Catalog No. ABIN3155731

Product Details anti-VDAC1 Antibody

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reactivity: Arabidopsis thaliana, Moss, Papaver, Spinach

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL), Blue-native PAGE (BN PAGE)

Cross-Reactivity (Details): No cross-reactivity with: Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms

Characteristics: Expected / apparent Molecular Weight of the Antigene: 29 kDa (for Arabidopsis thaliana)

Purification: serum

Immunogen: KLH-conjugated peptide conserved in all known higher plant VDAC1 proteins including Arabidopsis thaliana with the locus name: At3g01280

Application Details

Application Notes: Recommended Dilution: 1 : 5000 on 2-30 µg of protein/lane with standard ECL (WB), 1 : 500 (IL).
Cellular [compartment marker] of mitochondrial outer membrane

Comment:

Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-VDAC1 antibody (ABIN3155731)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).

Zhou, Zhao, Yang, Chen, Liu, Jin, Chen, Li, Deng, Schumaker, Guo: "Ubiquitin-specific protease16 modulates salt tolerance in Arabidopsis by regulating Na(+)/H(+) antiport activity and serine hydroxymethyltransferase stability." in: The Plant cell, Vol. 24, Issue 12, pp. 5106-22, (2013) (PubMed).

Takahashi, Teranishi, Ishida, Kawasaki, Takeuchi, Yamaya, Watanabe, Makino, Hidema: "CPD photolyase repairs ultraviolet-B-induced CPDs in rice chloroplast and mitochondrial DNA." in: The Plant journal : for cell and molecular biology, (2011) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC1 (VDAC1 Products)

Background: AGI Code: At3g01280
VDAC1 protein (called also Synonymes: At3g01280, outer mitochondrial membrane protein porin 1, T22N4_9, T22N4.9, VDAC 1, Voltage-dependent anion-selective channel protein 1, voltage-gated ion-selective channel) forms a channel through the cell membrane for diffusion of small hydrophilic molecules. Evolutionary origin of VDAC protein is not clear and their structure and properties are quite different making those proteins only conceptually like porins (Clausen et al. 2004).

Molecular Weight: 29 kDa (for Arabidopsis thaliana)

UniProt: Q9SRH5