GOGAT antibody

Catalog No. ABIN3155732

This anti-GOGAT antibody is a Rabbit Polyclonal antibody detecting GOGAT in WB. Suitable for Arabidopsis thaliana, Green bean, Miscanthus giganteus, Zea mays.

Quick Overview for GOGAT antibody (ABIN3155732)

Target: GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))

Reactivity: Arabidopsis thaliana, Green bean, Miscanthus giganteus, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-GOGAT Antibody

Characteristics: Expected / apparent Molecular Weight of the Antigene: 177 / 170-180 kDa depending upon the species
This antibody can be used as a plastidial marker

Purification: serum

Immunogen: KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7, At5g04140 and Fd-GOGAT 2 Q9T0P4, At5g41220

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB) MW 177 | 170 - 180 kDa depending upon the species.

Comment:

A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Target Details for GOGAT

Target: GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))

Background: AGI Code: At2g41220
Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95 % of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.

Molecular Weight: expected: 177 kDa, apparent: 170-180 kDa depending upon the species

UniProt: Q9T0P4