Heat-Shock Protein 101 (HSP101) (N-Term) antibody

Catalog No. ABIN3155733

Cited in 5+ publications. The Rabbit Polyclonal anti-Heat-Shock Protein 101 antibody (ABIN3155733) specifically detects Heat-Shock Protein 101 in WB. The antibody is reactive with Arabidopsis thaliana, Agave tequilana, Cabbage, Oryza sativa, Thellungiella salsuginea, Tomato, Triticum aestivum and Vicia faba samples.

Quick Overview for Heat-Shock Protein 101 (HSP101) (N-Term) antibody (ABIN3155733)

Target: Heat-Shock Protein 101 (HSP101)

Reactivity: Arabidopsis thaliana, Agave tequilana, Cabbage, Oryza sativa, Thellungiella salsuginea, Tomato, Triticum aestivum, Vicia faba

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Purpose: Anti-HSP101 | ClpB heat shock protein, N-terminal (rabbit antibody)

Cross-Reactivity (Details): Arabidopsis thaliana, Agave tequilana,Brassica oleracea, Oryza sativa, Solanum lycopersicum, Thellungiella salsuginea, Triticum aestivum, Vicia faba
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Dicots,including: Glycine max,Vitis vinifera,monocots

Purification: Serum

Immunogen: Recombinant Hsp101 N-terminal derived from the sequence of Arabidopsis thaliana Hsp101 protein P42730

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Mudrik, Kashentseva, Gamburg, Politov: "[Genetic passportization and identification of Siberian cranes (Grus leucogeranus Pallas) in captivity]." in: Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiĭskaia akademiia nauk, Issue 3, pp. 219-27, (2015) (PubMed).

Mishra, Grover: "Intergenic sequence between Arabidopsis caseinolytic protease B-cytoplasmic/heat shock protein100 and choline kinase genes functions as a heat-inducible bidirectional promoter." in: Plant physiology, Vol. 166, Issue 3, pp. 1646-58, (2014) (PubMed).

Fedoseeva, Rikhanov, Varakina, Rusaleva, Pyatrikas, Stepanov, Fedyaeva: "[Induction of Hsp104 synthesis in Saccharomyces cerevisiae is inhibited by the petite mutation in the stationary growth phase]." in: Genetika, Vol. 50, Issue 3, pp. 273-81, (2014) (PubMed).

Vainonen, Jaspers, Wrzaczek, Lamminmäki, Reddy, Vaahtera, Brosché, Kangasjärvi: "RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana." in: The Biochemical journal, Vol. 442, Issue 3, pp. 573-81, (2012) (PubMed).

Banti, Mafessoni, Loreti, Alpi, Perata: "The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis." in: Plant physiology, Vol. 152, Issue 3, pp. 1471-83, (2010) (PubMed).

Target Details

Target: Heat-Shock Protein 101 (HSP101)

Alternative Name: HSP101

Background: Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.

Molecular Weight: 101 kDa

Gene ID: 843771

UniProt: P42730