CD16 antibody

Catalog No. ABIN181096

This Mouse Monoclonal antibody specifically detects CD16 in FACS, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD16 antibody (ABIN181096)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DJ130c

Product Details anti-CD16 Antibody

Specificity: This antibody recognizes Human CD16, a 50-65 kDa cell surface molecule. It recognizes all polymorphonuclear cells irrespective on their NA phenotype. Studies show that the DJ130c antibody recognises an epitope in the first membrane-distal domain of CD16 and recognises both CD16A and CD16B.

Characteristics: Synonyms: IgG Fc receptor III-2, Fc-gamma RIII-alpha, FcR-10, CD16a, FCGR3A, FCG3, IGFR3, Fc-gammaRIIIa, FcRIIIa, Fc-gamma RIII, Low affinity immunoglobulin gamma Fc region receptor III-A

Purification: Affinity Chromatography on Protein G

Immunogen: CD34 antigen

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingheat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 isrecommended for this purpose. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: CD16 which is the low affinity receptor for IgG (FcRIII), exists as a transmembranous form (Fc gammaRIIIA, or CD16a) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16b). CD16a is expressed by NK cells, some T cells, and macrophages, whereas CD16b is primarily expressed by granulocytes. In addition, CD16b exists as two allelic variants NA1 and NA2.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process