CD3 antibody

Catalog No. ABIN316751

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS, IP and IHC (fro). It is suitable to detect CD3 in samples from Dog.

Quick Overview for CD3 antibody (ABIN316751)

Target: CD3

Reactivity: Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CA17-2A12

Product Details anti-CD3 Antibody

Specificity: This antibody recognises the CD3 cell surface antigen, expressed by mature T lymphocytes

Characteristics: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Purification: Purified IgG prepared by affinity chromatography on Protein G

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunoprecipitation. Flow Cytometry: 1/25 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containssodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled bytrained staff only.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 442981

UniProt: P27597

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway