Interferon gamma antibody

Catalog No. ABIN317387

The Mouse Monoclonal anti-Interferon gamma antibody has been validated for WB, FACS and IHC (p). It is suitable to detect Interferon gamma in samples from Cow.

Quick Overview for Interferon gamma antibody (ABIN317387)

Target: Interferon gamma (IFNG)

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7B6

Product Details anti-Interferon gamma Antibody

Specificity: This antibody recognises both native and recombinant IFN gamma.

Characteristics: Synonyms: IFN-gamma, gamma IFN

Purification: Purified IgG prepared by affinity chromatography on Protein G

Isotype: IgG1

Application Details

Application Notes: Western blot. Flow cytometry: 1/500 - 1/2000, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL (Membrane permeabilisation is required for this application, we recommendthe use of Leukoperm). Immunohistochemistry on paraffin sections (requires antigen retrieval using heattreatment prior to staining, Sodium citrate buffer pH 6.0 is recommended). Does not work in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

Gene ID: 281237

UniProt: P07353

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy