RYR1 antibody (C-Term)

Catalog No. ABIN317452

This Mouse Monoclonal antibody specifically detects RYR1 in WB, IF, EIA, IHC (fro) and FACS. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Pig.

Quick Overview for RYR1 antibody (C-Term) (ABIN317452)

Target: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This RYR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: G-1

Product Details anti-RYR1 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts with the C-terminus cytoplasmic domain of Ryanodine Receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. This antibody reacts with Ryanodine Receptor (~500 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).

Purification: Purified

Immunogen: Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodinereceptor

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1: 100 (Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescentmicroscopy will not be adequate to detect the RyR. Additionally, fluorescent, notenzymatic, detection is required. Due to the intensity of confocal lasers, use of ananti-fading agent, such as DABCO, is strongly recommended. ). Immunofluorescence: 1: 100. ELISA: 1: 1000. Flow Cytometry: 1: 500. Western blot: 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for RYR1

Target: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Alternative Name: Ryanodine Receptor 1 / RYR1

Gene ID: 6261

UniProt: P21817

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development