S1PR3 antibody

Catalog No. ABIN317744

The Rabbit Polyclonal anti-S1PR3 antibody has been validated for WB, IF and EIA. It is suitable to detect S1PR3 in samples from Human, Mouse and Rat.

Quick Overview for S1PR3 antibody (ABIN317744)

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-S1PR3 Antibody

Specificity: This antibody detects endogenous levels of EDG-3 protein.(region surrounding Phe155)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/40000-1/60000. Wetren blot: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for S1PR3

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Alternative Name: EDG-3 / S1P3

Background: EDG3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several protein tyrosine kinases. EDG3 is expressed in heart, placenta, kidney, and liver tissue.Synonyms: EDG3, Endothelial differentiation G-protein coupled receptor 3, FLJ37523, FLJ93220, LPB3, MGC71696, S1P receptor 3, S1PR3, Sphingosine 1-phosphate receptor 3

Molecular Weight: approx. 42 kDa

Gene ID: 1903

NCBI Accession: NP_005217

UniProt: Q99500