LPAR3 antibody

Catalog No. ABIN317747

The Rabbit Polyclonal anti-LPAR3 antibody has been validated for WB and EIA. It is suitable to detect LPAR3 in samples from Human.

Quick Overview for LPAR3 antibody (ABIN317747)

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-LPAR3 Antibody

Specificity: EDG-7 antibody detects endogenous levels of EDG-7 protein.(region surrounding Pro324)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LPAR3

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Alternative Name: EDG-7 / LPAR3

Background: The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.Synonyms: EDG7, Endothelial differentiation, GPCR, HOFNH3O, LPA-3, LPAR3, Lysophosphatidic acid receptor 3, Lysophosphatidic acid receptor Edg-7, lysophosphatidic acid G-protein coupled receptor 7

Molecular Weight: approx. 47 kDa

Gene ID: 23566

NCBI Accession: NP_036284

UniProt: Q9UBY5

Pathways: Regulation of Cell Size