CHSY3 antibody

Catalog No. ABIN317828

The Rabbit Polyclonal anti-CHSY3 antibody has been validated for WB, IF and IHC (p). It is suitable to detect CHSY3 in samples from Human, Mouse and Rat.

Quick Overview for CHSY3 antibody (ABIN317828)

Target: CHSY3 (Chondroitin Sulfate Synthase 3 (CHSY3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHSY3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHSY3 Antibody

Specificity: CHSY2 antibody detects endogenous levels of CHSY2 protein.(region surrounding Arg671)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CHSY3

Target: CHSY3 (Chondroitin Sulfate Synthase 3 (CHSY3))

Alternative Name: CHSY3

Background: Chondroitin sulfate synthases (CHSYs) synthesize chondroitin sulfate, a glycosaminoglycan expressed on the surface of most cells and in extracellular matrices. Glycosaminoglycan chains are covalently linked to various of core protein families and regulate many biologic processes, including extracellular matrix deposition, cell proliferation and recognition, and morphogenesis. The CHSY family includes CHSY1, CHSY2 and CHSY3. CHSY1 and CHSY3 display both glucuronyltransferase and N-acetylgalactosaminyltransferase activities, while CHSY2 is required for chondroitin polymerizing activity. CHSY2 localizes to the Golgi apparatus and is expressed ubiquitously, with highest expression observed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.Synonyms: CHSY2, CSS3, Carbohydrate synthase 2, ChSy-2, Chondroitin glucuronyltransferase II, Chondroitin sulfate synthase 3, Chondroitin synthase 2, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase II

Molecular Weight: approx. 85 kDa

Gene ID: 337876

NCBI Accession: NP_787052

UniProt: Q70JA7

Pathways: Glycosaminoglycan Metabolic Process