Cathepsin G antibody (Internal Region)

Catalog No. ABIN3183694

This independently validated Rabbit Polyclonal antibody specifically detects Cathepsin G in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Cathepsin G antibody (Internal Region) (ABIN3183694)

Target: Cathepsin G (CTSG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin G antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cathepsin G Antibody

Binding Specificity: Internal Region

Specificity: Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.

Characteristics: Rabbit Polyclonal to Cathepsin G.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cathepsin G.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA 1:40000,

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102721 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102721

Date: 11/05/2018

Antigen: CTSG

Lot Number: 022204892

Method validated: Unfolding Profile

Positive Control: ABIN3183694

Notes:

Passed. ABIN3183694 showed T_i at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Validation Images

p>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.

Full Methods

Primary Antibody: ABIN3183694

Full Protocol:

• Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Experimental Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for Cathepsin G

Target: Cathepsin G (CTSG)

Alternative Name: Cathepsin G

Molecular Weight: 22 kDa

Gene ID: 1511

UniProt: P08311

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones